Phlebovirus and Flavivirus detection in mosquito and sandfly populations and in FTA cards

Description: Current Phlebovirus (Bunyaviridae) and Flavivirus (Flaviviridae) related diseases are of global public health and animal sanitary interest. Viral and vector dispersion require multidisciplinary targeted strategies to prevent and control arboviral outbreaks in susceptible areas. Locations in various habitats at the Llobregat River Delta were chosen to develop mosquito (Diptera: Culicidae) and phlebotomine (Diptera: Psychodidae) captures with CO2 baited traps supplied with sugar baited preservation FTA cards. To test the efficiency of FTA cards in viral detection as a surveillance tool, a standardizing assay was developed evidencing high feeding rates and low mortalities in two species of laboratory mosquito colonies: Aedes albopictus and Culex pipiens. Generic RT ? nPCRs with degenerated primers and sequencing were performed for viral detection in female specimen pools and FTA cards. Species composition of the area showed Phlebotomus perniciosus as the dominant phlebotomine sandfly, and Culex pipiens as the most abundant mosquito species followed by the tiger mosquito Aedes albopictus. Insect Specific Flaviviruses (ISFV) were detected in an Aedes albopictus and Culex pipiens pools, confirming this flavivirus circulation in Catalonia. Further studies in FTA cards are neeed to confirm them as useful tool for surveillance programs.

Topic: EPIDEMIOLOG?A
VIGILANCIA ENTOMOL?GICA
VIROLOG?A
ARBOVIRUS
CULICIDAE
FLAVIVIRUS
PHLEBOTOMINAE
PHELOBOVIRUS
FTA CARDS
SUGAR-BASED
SURVEILLANCE

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