The effects of heat stress on the in vitro growth and development of bovine myofibers recovered from skeletal muscle
- Authors
- Valencia Gonzaga, Eduardo David
- Format
- MasterThesis
- Status
- publishedVersion
- Description
Heat stress (HS) is major issue for cattle industry, and projected future climate conditions may enhance the impacts. This investigation aimed to develop an in vitro model to assess the effects of increased temperature and betaine supplementation on the formation and development of bovine muscle cells. Primary skeletal muscle cells ?myoblast, were isolated from the vastus medialis muscle of two mature Hereford steers (H1 and H2) and induced to differentiation. A dose of 10mM of betaine wasincluded to differentiation media. Two different temperatures were used: 38.5?C ?TN?-control and 42.5?C ?HS?. Desmin staining was used to determine the purity of the myoblasts. Both animals had low levels of desmin positive cells H1 (58.40 ? 11.36) and H2 (20.10 ? 4.02). Creatine kinase (CK) activity was used to determinate myofibers differentiation. CK activity was found to decrease over time (days 2 and 4) in all treatments, meaning that myofibers were not formed. And the similar results were obtained when myoblast were cultured longer (days 6 and 8). The effect of betaine and increased temperatures could not be determined, since myofibers were not formed. Findings o f t his study s uggest that t he ? hanging drop? method is not effective to isolate bovine skeletal primary cells.
El objetivo de este estudio fue desarrollar un modelo in vitro para determinar los efectos de elevadas temperaturas y la suplementaci?n con Betaina en la formaci?n y desarrollo de c?lulas de m?sculo bovino.
- Publication Year
- 2015
- Language
- eng
- Topic
- BIOTECNOLOG?A ANIMAL
CULTIVO CELULAR
C?LULAS DE M?SCULO BOVINO
MODELO IN VITRO
- Repository
- Repositorio SENESCYT
- Rights
- openAccess
- License