Evaluaci?n de plantas medicinales como potenciales tratamientos anti-demencia usando el modelo Drosophila tau. Caso de estudio: ar?ndanos (Vaccinium spp.), t? verde (Camelia sinensis) y toronjil (Melissa Officinalis) en la enfermedad de Alzheimer
- Format
- MasterThesis
- Status
- publishedVersion
- Description
Alzheimer?s disease is the most common type of dementia accounting for 60 to 80% of cases. It is a degenerative brain disorder portrayed by continuous and progressive neuronal loss alongside ineffective connections between brain cells. There are no curative medicines and the few drugs used to alleviate the symptoms offer a varying degree of success and effectiveness. Therefore, it is imperative to find new and alternative treatments like phytotherapy which is a science-based approach to the use of natural products with medicinal purposes. Research has shown that blueberries (Vaccinium spp.), lemon balm (Melissa officinalis) and green tea (Camelia sinensis) have properties that improve brain activity in different ways placing makingthem a primary choice for investigation as natural treatments for Alzheimer?s Disease. In this study these three plants were tested to demonstrate their effectiveness inimproving the climbing ability of genetically modified Drosophila melanogaster flies that express the human Tau isoform as well as Oregon wild type flies. Fly food was supplemented with a dose of each one of the plants and one group was kept in standard fly food as control. Climbing assay were performed with Tau and Oregon flies at day 0, 2,4,6,8 and 10. Significant improvement over the control group was found in the climbing ability of the flies with blueberry (P=0.000) and green tea (P=0.000) supplementations. Lemon balm (P=0.836) performed better than the control but no statistical significance was achieved. The climbing ability of the wild type of flies was somewhat better than the control but with no statistical significance. Results suggest the compounds used should be further studied as potential Alzheimer?s Disease treatments.
La literatura a demostrados que los ar?ndanos (Vaccinium spp.), el toronjil (Melissa officinalis), y el t? verde (Camelia sinesis) tienen propiedades que mejoran la actividad cerebral en diferentes maneras. En este estudio estas tres plantas fueron probadas para demostrar su efectividad al mejorar la habilidad motora de moscas Drosophila melanogaster gen?ticamente modificadas para expresar la isoforma humana de Tau y en moscas Oregon de tipo salvaje. La comida de las moscas fue suplementada con una dosis de las plantas y un grupo fue mantenido en comida de moscas est?ndar para usarlo momo control. El ensayo de escalada fue realizado en los d?as 0,2,4,8 y 10. Una mejor?a significante estad?sticamente sobre el grupo control fue encontrada en las moscas suplementadas con ar?ndanos (P=0.000) y t? verde (P=0.000). El grupo suplementado con toronjil (P=0.836) mejoro su actividad pero no se logr? una significaci?n estad?stica. La habilidad de escalada de las moscas Oregon fue mejor que las del grupo control pero no hubo significacion estad?stica. Los resultados sugieren que los componentes usados deber?an ser estudiados m?s a profundidad como tratamientos potenciales para la enfermedad de Alzheimer.
- Publication Year
- 2014
- Language
- eng
- Topic
- PLANTAS MEDICINALES
ENFERMEDAD DE ALZHEIMER
FITOTERAPIA
DROSOPHILA MELANOGASTER
- Repository
- Repositorio SENESCYT
- Rights
- openAccess
- License