Characterization of F2C-FtsZ on DOPC/PE-MCC supported lipid bilayer

Description: We present the characterization of F2C-FtsZ (referred as Z2) on DOPC/PE-MCC supported lipid bilayer system. The characterization was performed using Atomic Force Microscopy (AFM) and Quartz Crystal Microbalance (QCM). AFM images were performed in the absence and presence of guanosine 5'-triphosphate (GTP). In the absence of GTP, Z2 protein on lipid bilayer images are visually indistinguishable from the lipidic bilayer images, but the image analysis shows that Z2 protein on lipid bilayer samples present a lower roughness than lipidic bilayer. QCM was unable to detect the adsorption of protein into the lipidic bilayer. AFM images in the presence of 10 mM GTP show a lament growth rate of 3.3 nm min 1 in average, the FtsZ laments grow in two directions, apparently led by the atomic structure of the mica. The characterized system can be used for studying FtsZ laments polymorphism, lament polymerization rates and polimerization led by solid substrates.

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