Comparison of the molecular methods, terminal restriction fragment length polymorphism and denaturant gradient gel electrophoresis, to characterize the microbiota in feces from breastfed infants

Description: Se aplicaron las técnicas “terminal restriction fragment length polymorphism” (T-RFLP) y “denaturant gradient gel electrophoresis” (DGGE) para caracterizar la microbiota intestinal de muestras de heces de siete lactantes durante sus primeros siete meses de vida. La comparación de los dendrogramas reveló diferencias entre los patrones de agrupamiento obtenidos usando los dos métodos. Estos resultados no concuerdan con hallazgos de estudios previos que han demostrado grandes similitudes entre los dendrogramas construidos a partir de muestras fecales usando las dos técnicas. Sin embargo, ambas técnicas fueron capaces de mostrar patrones de sucesión bacteriana y diferencias únicas en la composición de la microbiota de cada individuo estudiado.
Terminal restriction fragment length polymorphism (T-RFLP) and denaturant gradient gel electrophoresis (DGGE) were applied to characterize the intestinal microbiota in stool samples from seven breastfed infants during the first seven months of life. A comparison of dendrograms revealed differences between the cluster patterns obtained using the two methods. These results do not support the findings of previous studies that have demonstrated strong similarities between dendrograms constructed from stool samples using the two techniques. However, both techniques were able to show patterns of bacterial succession and unique differences in the microbiota composition of each individual studied.

Topic: Bacteriología - Técnica
Electroforesis
Lactantes - Heces Fecales - Investigación
CIENCIAS
MICROBIOLOGÍA