Bases para la domesticaci?n de Gaultheria Pumilla: estudio de diversidad gen?tica y protocolos de multiplicaci?n

Description: Polymorphic microsatellite markers were developed for Gaultheria pumila (Ericaceae) to evaluate genetic diversity and population structure within its native range in Chile. This is one of the most important Ericaceae species endemic to Chile. It has a large commercial potential and can be a very interesting target for breeding programs since it is very resistant to different abiotic conditions. Ten polymorphic Simple Sequence Repeat (SSR) loci were isolated from Gaultheria pumila using new-generation 454 FLX Titanium pyrosequencing technology. The mean number of alleles per locus ranged from 2 to 4. Observed and expected heterozygosity ranged from 0.00 to 1.0 and 0.00 to 0.64, respectively. From 10 SSR markers developed for G. pumila, nine markers are promising candidates for analyzing genetic variation within and between natural populations of G. pumila and other species from the same genus.

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