Ocurrence and diversity of endophytic colonization of taraxacum officinale by botrytis species: a preliminary study
- Authors
- Y?nez Navarrete, Zoila Elizabeth
- Format
- MasterThesis
- Status
- publishedVersion
- Description
The genus Botrytis (sexual form Botryotinia Whetzel) contains renowned plant pathogenic species with a necrotrophic lifestyle in which kill and feed on its hosts. Recent reports of symptomless endophytic colonization by Botrytis cinerea in cultivated plants of lettuce (Lactuca sativa) and Botrytis spp. in the wild plants dandelion (Taraxacum agg) and spotted knapweed (Centaura stoebe) reveal a different ?side? of Botrytis spp. These findings, expose additional strategies of Botrytis species to disseminate and grow in wild plants and also highlight the possibility that there exist (novel?) Botrytis species lacking virulence factors or with novel adaptations which could include some benefits to its hosts. In this work, the occurrence of endophytic colonization by Botrytis spp. in dandelion plants was investigated. The results, confirmed that symptomless dandelion plants were infected with Botrytis spp.. From these plants, 23 Botrytis isolates were obtained. By sequencing based on the genes HSP60 (Heat-shock Protein 60 ), G3PDH (Glyceraldehyde-3-Phosphate Dehydrogenase) and RPB2 (DNA-dependent RNA polymerase subunit II), 6 different genotypes were identified 3 of them showed the strongest phylogenetic association with B. cinerea whereas the other 3 were associated with B. pseudocinerea. Importantly, a pathogenicity test showed the capability of these isolates to cause disease lesions in tomato and Nicotiana benthamiana. In this report, the implications of the ecology of Botrytis species are discussed.
- Publication Year
- 2014
- Language
- eng
- Topic
- MICROBIOLOG?A
FITOPATOLOG?A
COLONIZACI?N ENDOF?TICA
NECROTR?FICO
DIVERSIDAD GEN?TICA
PAT?GENO DE PLANTAS
- Repository
- Repositorio SENESCYT
- Rights
- openAccess
- License
- openAccess